Tens of millions of Americans who work at companies with 100 or more employees will need to be vaccinated against COVID-19 by Jan. 4 or get tested for the virus weekly under government rules issued Thursday.

The Occupational Safety and Health Administration said companies that fail to comply could face penalties of nearly $14,000 per violation.

The new requirements, which were first previewed by President Joe Biden in September, will apply to about 84 million workers at medium and large businesses, although it is not clear how many of those employees are unvaccinated.

Tougher rules will apply to another 17 million people who work in nursing homes, hospitals and other facilities that receive money from Medicare and Medicaid. Those workers will not have an option for testing and will need to be vaccinated.

It was unclear how OSHA planned to enforce the rules. A senior administration official said the agency would target companies if it gets complaints.